10 "Breakthroughs" in Industrial Fermentation: These innovative solutions make high-density fermentation/continuous flow production no longer a challenge!
1. Strain selection and genetic engineering strain selection are the basis for improving fermentation efficiency. Through natural screening, mutagenesis, and genetic engineering methods, excellent strains that can adapt to specific environmental conditions can be cultivated.
Are bacteria selling at sky high prices? These 8 patented strains support the trillion dollar pharmaceutical market. What does the more expensive "bio chip" look like?
For example, using mutagenesis methods such as ultraviolet radiation and gamma rays, high-yield strains can be screened, and their metabolic capacity can be further enhanced through genetic modification.
In addition, optimizing the metabolic pathways of strains through genetic engineering can significantly increase the yield of target products.
2. Optimize fermentation medium
The composition of the culture medium directly affects the growth and metabolism of microorganisms. By using classic medium optimization methods (such as one-time factor method), experimental design (such as Plackett Burman design), and statistical medium optimization techniques, the composition of the medium can be systematically adjusted to improve the yield and quality of the product.
3. Fermentation process control and real-time monitoring
Modern fermentation technology emphasizes precise control over the fermentation process. By monitoring key parameters such as temperature, pH value, and dissolved oxygen in real-time, combined with an automated control system, the stability and efficiency of the fermentation process can be ensured.
4. High density fermentation technology
High density fermentation optimizes the stirring system, air distributor, and ventilation rate to enable efficient growth of microorganisms in high concentration culture media. For example, using an axial flow stirring system and an annular air distributor can significantly improve mass transfer efficiency and microbial growth rate.
5. Staged cultivation techniques
According to the metabolic characteristics of microorganisms, the fermentation process is divided into multiple stages for control. For example, providing sufficient nutrients in the initial stage to promote rapid growth, and increasing product synthesis efficiency by limiting nutrient concentration in the later stage.
6. Optimization of Reaction Dynamics Model
Optimization techniques based on reaction kinetics models can accurately predict and regulate the fermentation process by modeling and analyzing the stoichiometry, biological reaction kinetics, and transfer kinetics during the fermentation process.
7. Metabolic Engineering and Systems Biology
By combining metabolic engineering and systems biology methods, the metabolic network of microorganisms can be deeply studied, and their metabolic pathways can be optimized to improve the synthesis efficiency of target products.
9. Continuous flow fermentation technology
Continuous flow fermentation achieves continuous production by online supplementation of culture medium and removal of liquid phase products. This technology not only increases production intensity, but also reduces energy consumption.
